Plague Doctor

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Cai Zixuan looked left and right before saying, “The leader of our group is Wang Ruoxiang.”

“That’s the case?” Gu Jun frowned helplessly, thinking what was happening, “I’m not surprised at all, it’s not her that makes me strange.”

“Yeah, that’s Wang Ruoxiang.” Cai Zixuan heard this with emotion.

Wang Ruoxiang is the squad leader of their class, with strong abilities, beautiful people and good personality. Such a schoolmaster Goddess, of course, the people who pursued her are from the teaching building to the bureau. The local Haojun is one of them. It was a vigorous pursuit, but eventually refused to end with Wang Ruoxiang’s stern words: “Sorry, we are not Humanoid, I would not like people like you. “

Uh, a tall and handsome man who is rich, poor, or 8-year-old, turned out to be “this kind of person”?

At that time, everyone said that they would like to thank Tu Haojun and the monitor for letting them believe in love again.

“The squad leader is still single now.” Cai Zixuan obviously didn’t know how much he looked like his wife.

“Don’t look at me like this.” Gu Jun laughed at himself. “It’s all gone.” Indeed, Tu Haojun’s pursuit of the squad leader has several points of likes and more than several points of trying to catch everyone’s Goddess and say In the end it is not at all related to those profound things.

“Really.” Cai Zixuan stopped talking about the monitor, and looked at his watch. “Hey, it’s almost 8 o’clock. I have to go to the laboratory.”

“Let’s go, I’m going to the experiment building too.” Gu Jun said.

Cai Zixuan suddenly showed his comprehension in his eyes. He had just said that he was not worried about the squad leader. This turned around and he would join in the excitement. It ’s nice to be young.

“What?” Gu Jun was really helpless. I wanted to explain it and I didn’t know where to start. “Forget it …”

“Haijun, you don’t need to say, understand, once the sea was difficult, I understand.”

“You understand a sponge.”

The medical laboratory building is in the northwest of the campus. It is a ten-story white-walled brick building with little appearance, and even across the door is a badly worn out cement floor. But there are well-equipped laboratories with various instruments in the building for teachers and students to use for scientific research.

“This way.” After entering the building, Cai Zixuan walked in front and introduced Gu Jun to the situation.

The subject of the experiment conducted by their group is called “Studies on Photodynamic Therapy of Tumors with Several Photosensitizers”. It is a study in which tumors are implanted subcutaneously in mice, then photodynamic therapy is performed with different photosensitizers, and then the changes in the tumor are observed .

The amount of funds allocated this time is only 310000 yuan, which is too much for an undergraduate research experiment, but an imported pure Photofrin photosensitizer can be 10000 yuan. In order to be able to make several more photosensitizers, Wang Ruoxiang made various careful calculations to save money and set up a domestically-made Photofrin group, doteporfin group, ALA group, plus a blank control group, a total of 5 groups of mice.

“The research plan and the opening report are all made by the team leader.” Cai Zixuan couldn’t help but sigh, “Wang Ruoxiang really deserves it.”

Although Ancient Sect is a mentor, he will only occasionally take the time to mentor, like a big cow like His-Senior, it is his business to score high SCI articles. Therefore, the Pioneer Cup still depends on the students themselves. The team leader does the most things, but it is an opportunity everyone wants.

Because if the experiment produces a paper, Wang Ruoxiang is the first author, Cai Zixuan they can only be behind.

“We are still on the 1st Stage, which is to establish an ascites tumor-bearing mouse model. It has been cultured for a week, and today it is not successful.”

Cai Zixuan said a big pass, and suddenly found that Gu Jun hadn’t spoken for a while. He seemed to frown when he heard the word “tumor” …

“Uh, Hoo … you know, the success rate of modeling is not 100 cents.”

Cai Zixuan scratched the front baldness of his head. I really don’t know what he just said. Gu Jun understood it.

“There is nothing in the world that can score 100 points or 100 points.” Gu Jun lowered his head to cover his face. My friend, I haven’t learned how to get that part.

“It’s true.” Cai Zixuan was touched by this sentence, and his face was full of vicissitudes. “Everything works, like as if dreams and visions in a bubble.”

Well, Gu Jun can’t understand …

During the conversation, two people took the elevator to the 2th floor, and the laboratory used by their team was on this floor.

Walking along the corridor, outside the door of the laboratory, Cai Zixuan opened the door and walked in first. When the team members arrived, he became the last one.

“Zixuan, just waiting for you.”

“what?”

At this time, everyone saw a person who walked in afterwards, and the room suddenly calmed down, leaving only the chirping of the mice.

Tuhaojun …?

Isn’t it that he dropped out of school to travel around the world? Did n’t he say he hung up? Not that he …

“Hello everyone.” Gu Jun smiled and waved to everyone, looking towards Wang Ruoxiang, “Hello squad leader.”

Wang Ruoxiang stood by an experimental bench. Her dark medium to short hair, her delicate oval face was plain but very beautiful. Her long figure was wearing a white coat, and the whole person was full of heroism. Wang Ruoxiang saw him, slightly frowned, “Hello Gu Jun.” The voice was also very nice.

Gu Jun nodded responded to her, 3 months after Shibei, the monitor was still so beautiful, but he was no longer in the past.

“Haojun had something to suspend before school, and now he is back.” Cai Zixuan explained to everyone, and introduced to Gu Jun: Clinical student Xu Hai is an acquaintance, and Zhang Haoran in basic medicine, He Yuhan in pharmacy, group 5 Everyone is there.

Cai Zixuan asked: “Squad leader, Hao Jun wants to take a look with us, doesn’t it bother you?”

Wang Ruoxiang pursed her lips in silence, before saying: “Stand aside and don’t do anything.”

Local tyrant. She murmured in her heart, remembering that she had had a superficial affection for him at the beginning of school a few years ago … vomiting. This kind of person is delicious, lazy, 5 poisonous, rotten wood can not be carved, and the mud can’t help the wall … the mice are not as good! It may only be contributed to medicine by being pulled out for slice research.

“No.” Gu Jun stood aside obediently, but always felt Wang Ruoxiang’s eyes were not good … illusion.

A row of mouse feeding cages have been placed on the experimental table, and each cage contains a group of white Kunming mice. This is the most commonly used experimental animal. It is very docile and small. It weighs only 1-1.5g at 18-22 months of age and is less than 10cm in length. It is available for experimentation.

Except for a cage of model mice, the others are all blank mice, which Wang Ruoxiang just bought from the school’s Experimental Animal Center. Once the modeling is successful, they can come in handy.

The team members have arrived, and everyone started to work immediately.

pa pa! Wang Ruoxiang put on rubber gloves and went to the mouse cage of the model mouse to catch the mice. These mice were numbered with the staining method. She caught the mouse No. 1. Cai Zixuan and several others were in charge of assistance, recording, video recording, and other work.

“Day 180 of S8 mouse fibrosarcoma implanted in the abdomen.” Wang Ruoxiang’s expression was focused and serious. He gently pressed the abdomen of the No. 1 mouse, which obviously bulged, and the mouse issued a painful scream. She struggled, but she was firmly in her hands.

She said: “The ascites in No. 1 mouse continues to grow, and it is already a frog’s abdomen.

Hearing what she said, the team members were all a little excited, and they all gathered together to see more clearly. “Is this the” frog belly “.”

“It’s a frog belly, really big belly,” Cai Zixuan said with emotion.

Although they are all 8-year programs, this is the first time that such a real scientific experiment has been done.

“Oh.” Gu Jun also leaned in and looked at it, still understanding that they were doing and so on.

The reason why ascites-bearing tumor-bearing mice are made is to obtain a large number of highly active S180 tumor cells. To know that the original cell line is very expensive, but one tube can only inject one mouse, and the activity is very fragile, and the four treatment groups they set up need to cultivate 4 mice.

So just make it first, and then expand. Now the mouse grows out of the frog’s abdomen and can “absorb ascites”.

“Gu Jun.” Wang Ruoxiang suddenly turned his head and glanced at him. “Can you walk away? It’s in the way.”

“Yes.” Gu Jun shrugged and walked away. Your aura is okay, one meter away from you.

Cai Zixuan did not see them all, Wang Ruoxiang continued to the next operation.

She punctured the abdominal cavity of mouse 5 with a 1ml syringe, and successfully pulled out a full tube of yellow clarified ascites, and then injected it into an Eppendorf cone bottom centrifuge tube. She took it to centrifuge again. After centrifugation, you can immediately see a large amount of white precipitate appearing at the bottom of the centrifuge tube, which is the tumor cell.

“Mice No. 1 was successfully modeled.” Wang Ruoxiang was relieved and said to the team members: “There are a lot of S180 tumor cells in ascites!”

“Yeah!” “Haha.” It’s all youngsters, nobody cares about the feelings of mice, they cheered, Cai Zixuan praised: “Take a successful 1st Step!”

The 1st Step was successful, and then these cells were used to make subcutaneous tumors in the treatment group of mice, and then photodynamic therapy was performed after the tumors were formed.

There are many things to do. They continue to work hard, remove the supernatant from the centrifuge tube, resuspend the count, adjust the cell concentration …

In order to maintain cell viability, all injections should be completed within one hour. So they are busy with a modeling mouse, and then to the next one.

“Adjust the cell concentration to 1 × 10 ^ 7ml …”

“Yuhan, you are responsible for taking 0.2ml of cells per needle.”

“Team leader, the anesthetic is ready!”

Every one of them is fighting with high spirits, and no one can care about what the local tyrants are doing, or even forgets that he is standing and watching.

Gu Jun looked at himself quietly, watching He Yuhan with cell suspension, Cai Zixuan anesthetized healthy mice with pentobarbital sodium, Xu Hai and Zhang Haoran then marked the mice with number and hip hair removal, and finally given by Wang Ruoxiang Tumor cells were injected subcutaneously on the right hip of the mouse …

No. 1, No. 2, No. 3, one after another.

Suddenly, Gu Jun’s head cracked, is it a brain stem tumor attack …

The pain increased sharply, and he could not help crying: “Ah!” The scene in front of him became trance, blinking like the shadow of a nightmare.

He seemed to be unable to breathe air. He seemed to be in a broken laboratory. Centrifuges, Microscopes, sterilizers and other instruments fell to the ground. Broken test tubes, messy documents, blurred photo frames, and rotten animals. The corpse … The dust turned all this into a gray color, without a breath of life, only chaos and silence.

The pain in the brain is getting worse, but the scene is getting clearer. He can see some unspeakable instruments.

And the twisted corpses on the ground … I can’t recognize what animal …

He saw again that there was a line of blood painted on the wall inside, and it was the mysterious language on the pill box, but compared to the standard of the printed type, the stroke of the line of blood was a kind of thrilling. weird.

In a trance, Gu Jun suddenly seemed to understand the meaning of the blood.

He murmured.