Plague Doctor

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Zhang Lin took out the lifting cylinder from the liquid nitrogen tank, and then took out a freezing tube from the lifting cylinder, and then carefully put the lifting cylinder back, because there are many freezing tubes of other cells in the lifting cylinder. After Zhang Lin sealed the lid of the liquid nitrogen tank again, he began a series of patient and meticulous operations.

He first put the cryotube in a water bath, thawed quickly with hot water at 37 ° C, then changed the centrifuge tube and centrifuged for 5 minutes with a centrifuge, then sat down on the ultra-clean workbench, put the centrifuge tube tube mouth toward the alcohol lamp Bake and sterilize, discard the waste liquid, add fresh culture liquid with a pipette, and mix repeatedly by pipetting …

Gu Jun has been watching carefully next to him. If he has any questions, he asks Senior Brother Zhang to learn a lot.

Like the speed control of the centrifuge, the suction and discharge control of the pipette, etc., these sloppy recovery operations will affect the survival rate and proliferation rate of the cells.

Finally, Zhang Lin poured the cell suspension into the petri dish, added an appropriate amount of culture medium, and put it into a cell incubator at 37 ° C and 5% CO2.

“Wait, at least 2 days.” Zhang Lin took off his mask and pushed his glasses. “I suggest you keep it for a week, so that the rate of tumor formation is guaranteed.”

“It’s better to try a batch for 2 days.” Gu Jun said with a shrug. There was no way. The time was not enough. The system showed that he would get worse after 30 days. If it is true, maybe when the time comes even get out of bed and walk difficult. He said again: “Senior Brother, go out and rub it up at night? I treat.”

“No.” Zhang Lin waved his hand. “At night I’m going to feed the mice. I treat them.”

……

For the next 2 days, Gu Jun spent all on campus. In addition to gathering old with friends, one is to read a book to study and prepare for the make-up exam. If you ca n’t die but you have to repeat the grade, it ’s quite troublesome; 2 is to go every day. The experiment building takes care of the cells and replaces the culture medium once.

On the morning of the 3rd day, Zhang Lin personally looked at it and said that the cells had grown barely. He helped to subculture and prepare cell suspensions.

And Gu Jun went to the school’s experimental animal center, which is actually a large breeding base, located at several hundred meters away from the school park. It is lonely, probably because it smells of various animals.

He bought 5 nude mice, each with more than 100 pieces, which is quite expensive. Fortunately, he still has 2 10000 pieces of deposits, but it won’t last long.

When the money is used, I hate it less. Gu Jun really regrets donating the money so early … People are still alive, and the money is gone.

cough cough. Each of these nude mice has the size of 2 3 fingers and no hair. They are packed in a sterile box. Once they come into contact with the environment of bacteria, they can easily be infected and die. Therefore, the usual feeding and experimenting processes need to be carried out in a barrier environment.

Dongda ’s Barrier Environmental Laboratory is located in the Laboratory Animal Research Center. This 5-Layer building with yellow brick walls stands next to the experimental building.

Gu Jun and Zhang Lin met in the lobby of the first floor of the research center and booked a small nude mouse room for one hour. This is a small room of only 21 square meters, divided into the front room and the back room, the front room has experimental benches and instruments, and the rear room is the breeding room.

Before going in, they and all the items they carry must undergo strict ultraviolet sterilization. 2 people set foot on the nude mouse room with a full-length protective clothing, masks, gloves, foot covers and other special equipment.

Soon, on the anterior laboratory bench, Gu Jun marked five nude mice with a punch, and then injected them with tumor cells subcutaneously on the right side of the buttocks.

Zhang Lin is responsible for assisting next to him. These cells hit humans, in theory, they can also form tumors. Seeing that Tu Haojun was so enthusiasm for the nude mice, he was sure that the little brother did not want to play murder.

After all 5 nude mice were injected, Gu Jun put them in the feeding cage, and then it was up to them to see if they could form tumors.

“Senior Brother, many thanks.” Gu Jun gave Senior Brother Zhang a thumbs up. Without Senior Brother’s help, it would not be so smooth.

“The old Chinese doctor you mentioned …” Zhang Lin still looked at Conan’s alert look, “Is it really a liar?”

……

The following week, Gu Jun continued his previous life rhythm, but only went to the nude mouse room every day to see the caged nude mouse. Their breeding is under the unified care of the management staff of the nude mouse room, and each room and board costs 3 yuan per day.

This week, he only completed 3 common tasks, because those who need to cure and save people can’t do it. Although the hospital is nearby, he is not a doctor. The tasks he completed were all related to medical exercises. There were already 5 boxes of pills in his hand, and the proficiency of Tairan’s hand reached First Layer (30005000).

On the 7th day, Gu Jun walked into the nude mouse room again with sterile equipment and observed the nude mice one by one.

它们都没有死，1号的臀部右侧皮下已经长出一颗黄豆般大小的类圆形肿瘤，经测量，直径为0.5cm；3号和4号也有明显的突起，直径分别是0.3cm和0.5cm；2号和5号则仍然没有任何成瘤迹象。

So far, he can be sure that 5 nude mice and 3 tumors.

Gu Jun set 2 tumor-bearing nude mice as group A, one tumor-bearing nude mouse as group B, 2 non-tumor-forming nude mice were divided into group C and group D, and went to the animal center to buy 10 healthy nude mice , 5 set E group, 5 set F group. Group A, Group C and Group E will all receive the same dose of “different medicine” (he named) every day; Group B, Group D and Group F will not have any administration, only for control purposes.

That is, tumor-bearing nude mice, non-tumor-bearing nude mice, and healthy nude mice each have a group of medication, and a group does not.

At this point, the six cages had been divided on the experimental platform, and the nude mice chirped.

Gu Jun fiddled with 25 xenobiotic capsules. Since the system says that a box can extend lifespan for 5 days and a box of 5 capsules, it should be one capsule a day. This is the dose for adults, and the average weight of these nude mice is less than 20g. If they also take one capsule a day, it is estimated that they will be sacrificed immediately.

He poured out the medicine powder in a capsule and weighed the dose. He then converted the dose of nude mice to his body weight, formulated it into a solution, and diluted it proportionally.

Soon, Gu Jun held the gastric lavage needle with the potion in one hand, grasped a healthy nude mouse in one hand, and started the first intragastric administration.

“Haw.” The nude mouse struggled a little, but this poor thing was still pushed by him with this medicine and made its contribution to medicine.

At this moment, Gu Jun’s heartbeat was a bit fast, and his eyes were fixed on the naked mouse in his hands without blinking.

He had never done a toxicity test of foreign medicine until this moment. If it died abruptly, perhaps this experiment would be meaningless. And now, he stared at it for 5 minutes, followed by 10 points …

Half an hour later, the nude mouse still showed no abnormalities.

“It seems that there is no obvious acute toxicity of the foreign medicine.” Gu Jun breathed a sigh of relief. “The chronic toxicity has to be observed again.”

Then he continued to administer the medicine to a nude mouse. After taking 3 minutes to complete the administration of the other 7 nude mice in the 1 dosing groups, he observed for another hour. They all showed no abnormal reaction.

“Dear friends, let’s live.” Gu Jun murmured, pressing his painful head, his condition was obviously getting worse, “Please.”

……

On 2nd day and 3rd day, Gu Jun continued to work every day in the nude mouse room.

The tumors of the nude mice in group B that have not been administered have increased from 0.3 cm to 0.6 cm, and their body weight and mental state have not changed; while the two nude mice in group A who have been administered have the size of the tumor after 2 days. No change, maintained at 2cm.

Gu Jun knows what this might mean, the xenobiotics are really effective! These capsules can significantly inhibit tumor growth.

And the other administration of nude mice is as usual, which also means that the foreign medicine seems to have no adverse reactions and does not damage the body’s function much.

“Maybe, this time it’s really saved?” He thought, although the restlessness was still entangled in his heart, a new hope burned at the same time.

Day 4, Day 5 … In a blink of an eye, a week passed.

The tumor of the nude mice in group B has grown to 1.5 cm. The huge tumor is in sharp contrast with its thin body. It has started to lose weight and its spirit has become bad. However, the two nude mice in group A continued to be in stable condition, and all the nude mice administered were living well, which was no different from the blank control group.

I have to say that the results of this experiment are too satisfying.

The medicinal efficacy of the xenobiotic is better than that of the top tumor small molecule targeted medicine currently on the market.

However, Gu Jun also knows that the adverse reactions of foreign medicines cannot be completely ruled out, which just proves that it has no side effects in a short time. And this is an animal experiment after all, and there are certain differences from human clinical experiments.

However, the results of this experiment are already worth his risk.

This afternoon, after Gu Jun wrote his suicide note, he came to the waiting area of ​​the emergency department of Dongda Affiliated Hospital and sat down in a rare waiting seat. Surrounded by patients and family members of the vast crowd, mostly crying children and coaxing parents, nurses hurriedly walked around, nurses pushed the rescue bed …

Noisy, the emergency department broadcast continued to call: “No. 182, Chen Ziyang. No. 183, Wang Yuxuan. No. 184, Huang Zixuan …”

After exhaling, Gu Jun threw a capsule of the xenobiotic into his mouth and swallowed it with a sip of mineral water.

“Come on.” He looked calmly and looked at the emergency sign on the wall over there. “If there is any toxic reaction, come all.”