The Science Fiction World of Xueba

Pang Xuelin paused, and continued: "At the time when the virus leaked from the Hive Lab, I was in a hurry and injected a sample of the new virus into my body, and I didn't have time to copy the relevant gene sequence of the new virus from the Hive Lab. , so now it is very difficult to re-develop that virus, and there is another problem..."

"what is the problem?"

"The Kempten base laboratory is relatively simple, and we lack some high-end experimental equipment and equipment. For example, if we want to measure the dynamic characteristics of proteins now, we need dynamic APT, which is the so-called dynamic atom probe chromatography. Through it, we The dynamics of protein molecules as well as RNA molecules can be observed."

Albert pondered for a moment and said, "You make a list of the materials you need, and I will send someone to help you get all the equipment in place."

Pang Xuelin showed a satisfied smile on his face and said, "Mr. Albert, I will trouble you then."

Next, Pang Xuelin cooperated with the researchers brought by Albert Wesker and let them collect relevant blood and biological samples from himself.

He even called Alice and asked her to cooperate with the umbrella company in collecting samples.

Now because of the appearance of Pang Xuelin, Alice is no longer the focus of the umbrella company, and Albert has no opinion on Alice's appearance at the Kempten base.

After all these tasks were finished, Albert Wesker declined Pang Xuelin's invitation to have dinner together, and hurriedly boarded the plane to leave.

Watching the Umbrella Company's helicopter going away, Pang Xuelin said to Alice who was following him: "Alice, get ready. After the Umbrella Company's equipment is in place, you can go to Hill Military Base."

"good!"

Alice nodded.

Two days later, Umbrella Corporation delivered the Dynamic Atom Probe Chromatograph and various cutting-edge experimental instruments and consumables.

Spruce, Berkeley, Huang Yu and others were stunned. Many of the experimental equipment here are unique to Umbrella Company, and the outside world has never seen it.

Even Pang Xuelin watched the dynamic atom probe tomography instrument for a long time. If this thing is put into the modern society, it will be regarded as a big killer for both biology and material science research.

It's just a pity that he is now mainly focused on studying how to fight against the T-virus, and has no time to learn APT technology at all.

A week later, Alice led an elite team of more than 200 people to sweep the Hill base at the expense of more than 20 people, and obtained a large amount of weapons and ammunition from the Hill base.

In addition to rifles, machine guns, armored vehicles and various artillery, Alice actually brought back three tilt-rotor helicopters.

In this way, the external long-distance transportation of the Kempten base will no longer rely solely on the fleet of the umbrella company, which is of great significance to the future development of the Kempten base.

In addition, Alice did not disappoint Pang Xuelin, and even got three tactical nuclear warheads at Hill Air Force Base.

However, Alice did not transport these nuclear warheads back to the Kempten base, but hid them in a valley 20 kilometers away from the Kempten base.

Only Pang Xuelin, Alice, Claire, and Jill knew about this matter. These three nuclear warheads had a small yield, only equivalent to the power of 20,000 tons of TNT, and the weight of a single warhead was only about 50 kilograms.

Pang Xuelin can walk like flying in the mountains with two nuclear bombs in hand.

The secret bases of the Umbrella Company are basically located underground. If nothing can be done, or Spencer puts pressure on the Kempten base after realizing that Pang Xuelin has been perfunctory himself, these three nuclear warheads are enough for Pang Xuelin to destroy Spencer. Spencer's underground base.

In the middle of the night, after confirming that the three nuclear bombs were hidden, Pang Xuelin and Alice returned to the Kempten base quietly.

"Okay, it's getting late, you should go back and have a rest early."

Pang Xuelin was about to say goodbye to Alice and go back to his room to rest.

Alice suddenly said, "Wait..."

"What's wrong?"

Pang Xuelin turned around, looked at Alice and said.

Alice said, "Pang, when will we attack Umbrella Corporation?"

Pang Xuelin was slightly taken aback, and said, "Didn't it be agreed that I will do it after I develop an antidote against the T virus?"

Alice shook her head and said, "I'm afraid I can't wait that long."

"Why?"

Pang Xuelin frowned.

Alice said: "Spencer wants to destroy all human beings to build his super empire. It has been nearly a year since the outbreak of the T-virus. At present, the total number of surviving human beings in the world is less than 10 million. There must be, and in a few years, even if we kill Spencer and the others, there may not be many humans left on Earth."

Pang Xuelin said: "Then what are you going to do?"

Alice said: "In one year, within one year, if you can get the antidote for the T virus, then we will continue to cooperate. If you can't, I will bring a nuclear bomb to talk to Spencer."

Pang Xuelin looked at Alice quietly, he could feel the other party's determination.

Pang Xuelin pondered for a moment, then said: "Okay, then I will try my best."

Alice just nodded lightly, stopped talking, and turned to leave.

Pang Xuelin looked at Alice's back, feeling a little helpless.

Although he fooled Alice to the Kempten base, and the two had been together for half a year, he could feel that Alice didn't have complete trust in him.

However, there is no way around this. Pang Xuelin is still making false claims with the umbrella company.

If Spencer hadn't been more interested in the gene optimizer in Pang Xuelin's body instead of attacking Alice, I'm afraid Alice would have gone berserk long ago.

...

Alice's words put a certain amount of pressure on Pang Xuelin, and in the following time, he devoted all his energy to the research on the antidote to the T virus.

Now, it was the turn of Spruce, Ashford, and Berkeley to be shocked.

Although Pang Xuelin followed them to study the T virus before, he played the role of an assistant most of the time and rarely expressed his personal opinions on academic issues.

But when he really started to lead the operation of the laboratory, he quickly showed the strength of an academic bull.

There are a total of more than 30 people in the laboratory. Except for Pang Xuelin, Spruce, Berkeley, Huang Yu, and Ashford, the rest are doctoral students with a certain academic background like Xiaobei. Or a research assistant.

During the first three months of the study.

Pang Xuelin first asked Xiao Dongbei and the others to analyze the structures and data of various proteins and non-coding RNAs in cells through cryo-electron microscopy, atom probe chromatography, nuclear magnetic resonance and other equipment.

After obtaining these data, Pang Xuelin began to use the mathematical method controlled by MOSS to establish the dynamics and biological function models of the structural and dynamic characteristics of proteins and non-coding RNAs, and asked MOSS to write simulation software for proteins and non-coding RNAs.

This work is cumbersome and complicated, but Pang Xuelin insisted on relying on his strong mathematical foundation and the super computing power of quantum computers to complete the software writing work.

When he applied this software to the actual work, he instantly stunned Spruce and the others.

Through this software, scientists can actively construct some new RNA molecules and protein molecules, and then simulate the biological functions of these molecules through software.

As a result, they soon discovered that this software not only simulated with extremely high accuracy, but could even predict the existence of some undiscovered protein molecules and RNA molecules.

Even, Pang Xuelin used this software to build a biodynamic model of the T virus, perfecting the picture of the T virus invading the human body.

First, the T virus binds to the specific receptor of the host cell through its surface glycoprotein, and then the viral envelope and the plasma membrane (that is, through a pH-independent pathway) or the nucleosome membrane in an acidic environment (a pH-dependent pathway) After fusion, the viral ribonucleoprotein complex (RNP) is released to the cytoplasm, and each mRNA is synthesized during the transcription process, and the full-length antisense genomic RNA is produced through replication, which serves as a template for the viral genomic RNA.

The newly synthesized RNP complex is assembled with viral structural proteins in the plasma membrane or Golgi membrane, and then the newly synthesized progeny virus is released.

The progeny virus grows and reproduces in the cell, and then launches a large-scale attack on the surrounding cells.

Moreover, the T virus also has a magical feature. The RNA in its body can not only use normal human cells to reproduce, but can even form a linkage effect with some non-coding RNA in the human body, changing the growth and metabolism of human cells on a large scale.

What Pang Xuelin and the others have to do is to find a non-coding RNA that can interfere with the RNA of the T virus, so that the T virus will fall into a silent state. Even if it infects the human body, it cannot be expressed in the human body because the gene cannot be expressed. reproduce.

...

Time goes by day by day, and before you know it, half a year is fleeting.

On this day, Pang Xuelin had just arrived at the laboratory, and Spruce, who was on duty, excitedly found Pang Xuelin, and said with a big smile, "Professor Pang, have we found a target RNA?"

"Found it, which group is it?"

Pang Xuelin's eyes lit up, and he said in surprise.

Using the protein and RNA molecular biodynamic function simulation software developed by Pang Xuelin before, Pang Xuelin and the others wrote a special RNA that can silence the T virus gene.

Then, they screened thousands of RNAs in human cells and found more than two hundred kinds of RNAs.

Next, it is a group of experiments in the past.

This kind of work is very tedious, basically just repeating the previous work over and over again.

Pang Xuelin did not take the initiative to participate in it. He has already planned the follow-up research route, and is only waiting for the day when the target RNA is found.

It’s just that during this time, the laboratory has experimented with hundreds of non-coding RNAs, but has not been able to achieve the desired effect.

Pang Xuelin wondered if his previous path was wrong. If Alice really wanted to leave Kempten Town and embark on the road of direct confrontation with the umbrella company when the one-year appointment time came, Pang Xuelin really had no good way to stop it. other side.

Spruce said: "It is the 112th group. After we injected this active RNA extract into the cells, we observed a significant reduction in the T virus content in the cells through dynamic APT, and even stopped the synthesis of the RNP complex. .”

Pang Xuelin nodded and said with a smile: "This is good news. Have you observed how non-coding RNA No. 112 achieves gene silencing of T virus RNA?"

Spruce said: "We found that after RNA No. 112 enters the cell, through the RNA-protein linkage in the cell, the lysine residues in the N-terminal domain of histones can be deacetylated and methylated. Modification, this process is catalyzed by methyltransferase, the modification can be monovalent, divalent and trivalent methylation modification, T virus RNA can form heterochromatin with protein combined with methylated modified histone, in In the above process, in addition to the deacetylation and methylation modification of the N-terminal tail domain of some histones, sometimes it is also necessary to modify the lysine or arginine residues of the N-terminal tail domain of other histones. The acetylation modification is carried out accordingly, and the final result of this modification is that the gene silence of the corresponding segment of the T virus loses transcriptional activity..."

Pang Xuelin pondered for a moment, and said, "Bring Dr. Ashford, Professor Huang Yu, and Professor Berkeley, and we will hold a closed-door meeting to study the follow-up work plan."

"good!"

Spruce nodded.

Finding the target RNA is just an .asxs. There is more important work to be done next.

What Pang Xuelin wants is not only to make human beings immune to T virus, but what he wants to do is how to use this target RNA to create a new type of airborne virus, which is basically equivalent to a wireless virus in the human body. It is a harmful endogenous retrovirus, but in the bodies of zombies and people infected with T virus, it can instantly inactivate the T virus in their bodies.

In this case, when they deal with Spencer and his men, they will lose all kinds of superpowers given by the T-virus, so Spencer and the others are just a group of chickens and dogs in front of Pang Xuelin.

This kind of work requires the collective efforts of Ashford, Berkeley, and Huang Yu.

Sure enough, after learning that the target RNA had been found, Ashford and Berkeley showed joy on their faces.

The discovery of target RNA gave them a ray of hope in this dark age.

However, using target RNA to assemble a new virus is not a simple task. Pang Xuelin, Spruce, Ashford, Berkeley, and Huang Yu discussed for a whole week before completing the setting of the target virus.

Next, they combined this RNA with adenovirus (AAV, as mentioned earlier, this virus is harmless to the human body and exists in many people, and scientists often use adenovirus to make vaccines), and then used A new type of gene editing tool adds some genes to the adenovirus, making this new type of virus capable of air transmission.

At this time, there is only less than a month left before the agreement between Pang Xuelin and Alice.